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When and How to test for Syphilis

Syphilis Test

Phew. Finally found the time to sit down and pen this long awaited entry. I have been getting questions about Syphilis testing and would like to set the record straight.

Syphilis Symptoms

First of all a few basic facts. Syphilis is a bacteria. It is transmitted via direct skin contact with contagious Syphilitic lesions. What are considered contagious Syphilitic lesions you say? Well, they are ulcers caused by Syphilis (known as a Chancres) and the skin rash that occurs in secondary Syphilis. The ulcers are generally single and painless (except in HIV positive patients where the ulcers can be multiple and painful). They can appear anytime between 9 to 90 days post infection. Most commonly they appear at about 4 weeks post infection. The ulcers are found at the site of bacterial entry. So they can be anywhere but they are most frequently found in the anogenital region. The rash caused by secondary Syphilis is called the Great Imitator because it can look like any rash. It appears 3 to 6 weeks after the initial Syphilitic ulcer (Chancre) has subsided. Since it can look like anything, it is hard to differentiate from other rashes (eg eczema). It is usually a macula-papular rash that involves the trunk more than the limbs and also involves the palms and soles.

At this point some of you are asking ‘If what you say is true, then Syphilis is not necessarily sexually transmitted.’ To that I say ‘You are absolutely right.’ There was a user on the forum (www.askdrtan.com) who caught Syphilis from her client. She is a masseuse. Direct skin contact with infectious lesions remember?

Syphilis Stages

Next I want to clear up some technical terms. Often people throw words at me like ‘Early Primary Syphilis’ and ‘Latent Secondary Syphilis’. This shows that there is a lot of confusion in the terminology out there. So let’s set the record straight.

There are 2 stages of Syphilis: Early and Late. The definition of early Syphilis VARIES depending on which guidelines you read. According to the US CDC, if the Syphilis infection was within the past 1 year, it is considered Early Syphilis. According to the WHO it is 2 years. Why does this matter? It determines what treatment a patient gets. Early Sypphilis requires less medicines than Late Syphilis. So far confusing enough? It gets worse.

Early Syphilis is divided into 3 clinical stages. Primary Syphilis, Secondary Syphilis and Latent Syphilis (also known as Early Latent Syphilis). The stages are characterized as such: Primary Syphilis – presence of Chancre, Secondary Syphlis – presence of rash, Latent Syphilis – no symptoms.

Late Syphilis is divided into 2 clinical stages. Tertiary Syphilis and Latent Syphilis (also known as Late Latent Syphilis). The stages are characterized as such: Tertiary Syphilis – End organ involvement including aneurysms, Latent Syphilis – no symptoms.

There are 2 other types of Syphilis we will not be discussing in this post and they are Neuro-Syphilis (Syphilis involving the nervous system) and Syphilis in the Newborn.

Syphilis Tests

Remember we said Syphilis is a bacteria? To be more precise, Syphilis is the disease caused by a bacteria and this bacteria is called Treponema Pallidum. The special thing about T. Pallidum is that it cannot be cultured. In other words, you cannot grow it in a dish. The only way to prove it is there is either to see the live bacteria directly or by using indirect evidence of its presence (something known as serology tests which are essentially blood tests).

To see the live bacteria you first need an active lesion. Next, you need a skilled person to sample bacteria from the lesion. Next you need special equipment and specially trained medical professionals to use this special equipment to look for the bacteria. Because the case load of Syphilis for most countries is rather low, most labs either do not have the equipment or the training. Also, because if the high rate of false negatives from these tests, patients suspected of Syphilis are required to follow up with serology testing anyway. In other words, these tests are rather useless except in very specific settings.

Serology tests are the mainstay of diagnosing Syphilis. There are basically 2 types of serology tests: Treponemal Tests and Non-Treponemal tests. There are many types of Treponemal Tests (eg TPHA, FTA-Abs etc). There are also many types of Non-Treponemal tests (VDRL, RPR, HEAT etc). For ease of discussion we will consider all Treponemal tests the same and all Non-Treponemal tests the same.

The one very important thing to remember is that there is no such thing as one perfect ‘most accurate’ test for Syphilis. This is what most people look for and it is really just chasing a rainbow.

Treponemal tests are very good at screening for Syphilis. They are as good as 100% accurate in diagnosing Syphilis except under 3 conditions. The first is very early Syphilis. When I say very early I mean less than 12 weeks from the date of infection. Although Treponemal tests are as good as conclusive at 4 weeks, the recommendation is still to retest at 12 weeks. The second is very late latent Syphilis. How late? Nobody knows. The accuracy of Treponemal tests is late latent Syphilis drops from 100% to 97% over several years. The third and last condition under which Treponemal tests are of no use is if a person has been previously infected with Syphilis. Even if this person has been treated successfully, the Treponemal test will still remain positive. So there is no way to know if this person has been re-infected or not using Treponemal tests.

Non-Treponemal tests are often used for screening of Syphilis in places where the prevalence of Syphilis is relatively high. This is because of the third reason given in the preceding paragraph. (Also because it is cheaper). So you would notice that European Guidelines recommend using Treponemal tests for screening and most Asian guidelines recommend using Non-Treponemal tests. On the whole, Non-Treponemal tests are LESS ACCURATE than Treponemal tests when used for screenig of Syphilis. They are however useful under 2 conditions. The first is when a person has been previously infected with Syphilis and we need to find out if he or she has been re-infected or not. The second is for monitoring of treatment success or failure.

So remember we said there is no one perfect test for screening of Syphilis? The trick is to do both a Treponemal as well as a Non-Treponemal test for patients. That is ideal.

If a patient has never had Syphilis before, which would be the majority of readers here, and you HAD to choose one test, than the Treponemal test is better. Remember to always test at 4 weeks and retest at 12 weeks.

I hope that added some clarity to Syphilis testing.

I would like to end off on the note that nothing in Medicine is straightforward. Just when you think you had a handle on Syphilis testing, let me tell you this: All the above serology tests mentioned above will also be POSITIVE if a person has any of the Non-Syphilitic Treponemal Diseases. You didn’t think Syphilis was an only child did you? There are other bacteria under the Treponemal family which will all test positive using the exact same tests as we use to diagnose Syphilis.

So after all this discussion, we come back to square one. The same usual boring advice I give everyone.

Do not try to be your own doctor by reading the internet. If you want to know whether you have been infected with Syphilis or not, please Our Doctors trained in such matters and heed his advice.
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About Dr. Tan
Dr. Tan graduated from the National University of Singapore in 2001. His residency was in the two largest public hospitals in Singapore; Tan Tock Seng Hospital and Singapore General Hospital.

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  1. 4 weeks post exposure tested with TPHA , VDRL (RPR) non reactive.had a red lesion on meatus.my dr says forget about it as its not syphilis.are my tests conclusive?

  2. Hi Dr,

    I was treated for secondary syphilis 3 months after infection. I received two penicillin injections. The following day after i received treatment I had eye sight pressure a floating white spot in left eye. Is this related?

    • It does not sound related, but I cannot really comment without evaluating your symptoms and condition. If you are still concerned, please speak to your doctor or visit us for a proper consultation.

  3. Exposed 24th July 2018 and tested on 14th sept 2018 (7 weeks)
    VDRL(RPR) serum, (slide floculation) – non reactive

    TPHA – negative

    Please advice me result conclusive?
    Or further test require?
    Today 16 th sep 2018 noted v shape red bump on my bottom of tongue!! Any relation with syhipilisi
    Pls advise

    • I would generally consider your testing so far to be conclusive, but I cannot comment on your tongue lesion without a proper evaluation. If you are still concerned, please visit us for a consultation.

  4. Kyran

    Dear sir I had RPR and TPPA tests done at around 112 days after exposure both came back negative. Now around 130 days I am having small bumps on my palms…I am bit worried now … should I do a retest….

    • I’d consider your tests conclusive at that point already. If you are concerned, please have a doctor evaluate the lesions.

      • Kyran

        Thank you sir. I will consult the doctor. Another RPR qualitative was done today and it is non reactive. Does this suggest its not syphilis…and something else?… should I consult a dermatologist…?

      • Kyran

        Thank you sir. I will consult the doctor. Another RPR qualitative was done today and it is non reactive. Does this suggest its not syphilis…and something else?… should I consult a dermatologist..

  5. Brick9799

    Doc. How good is a Syphilis TP CMIA blood test at 7 weeks after exposure?

    • Dr. Julian Ng


      Generally, if you were infected with Syphilis, most of the time it would have become positive after about 1 month. However, you may want to consider re-testing at 3 months.

  6. Hi

    I’ve been offered a new job in Singapore!
    I’m currently residing in the UK.

    I have been treated since 2012 from 1:64 to currently 1:4 in 2018

    Will I loose my right to work there and have my ep cancelled

    Is there anyway I can explain the situation to the mom?

    I would very much appreciate any help or advice

    Thank you.

    • Dr. Julian Ng


      Generally, if you have documentation from a medical practitioner in UK who can certify that you have treated and repeat testing shows 4-fold decrease in the titers, you can use that as your supporting documentation to MOM. Decision on your EP is at the discretion of MOM and thus I’m unable to advise you on that. My suggestion is to send an enquiry to MOM to clarify the situation.

  7. I believe I was infected with Syphilis somewhere between 5 and 12 years ago as I realise now I’ve gone through the primary (sores), secondary (rash, sore throat where you feel like you’re getting sick but never do), latent stages and am now experiencing organ issues as a result – I have never been tested but need to desperately figure this out – will a blood test confirm I have Syphilis?
    Thanks in advance for your help!

    • Yes, a blood test is enough to confirm a diagnosis of syphilis. Please visit us for testing and treatment.

      • my syphilis test is positive, HIV is negative. Tests done at anonymous clinic her in SG. Working and holding long term work permit. Will my work permit be cancelled? The councillor took my details including IC when my test is positive.

  8. Anonymous

    I suspect I might have syphilis, can I get treated as precausion ?because I’m on the early stage as per the exposure timings from Feb 1st week. Also, I’m taking pep, does it have any impact?. I want to get treated because some of the posts said that sti with hiv may increase the viral load on body.

  9. Emma White

    I got a white tongue ten days after unprotected oral sex. The tongue was painful for three days then all pain went away. I tested negative for all stds and HIV in Week 2, 3 , and 7.I am wondering if you think it could be syphilis or hiv? I still have the white tongue but think there could be false negatives or I tested too soon. Can you tell me?

  10. Ashton B Fairy

    Hello there!

    I had sex with someone on December 16. I tested negative for hiv and syphilis and everything else on December 26, 28, and again on February 9th.
    At this point it was almost 8 weeks later. What are the chance of false negatives? I used a condom but gave oral. I also have a white coated tongue. But tested negative for yeast.

  11. Hai doctor I had test for Syphilis VDRL method negative but government hospital they test VDRL positive 1:4 and tpha positive but last time when I have sex with some one using condom that also six month Back any possible for old infection

  12. Hi Dr Tan . what if a person have an exposure 10 years ago and recently thinking to get std and hiv test. Will the test be able to pick up if he or she has it ? Since it has been so long after exposure

  13. Hello dr.
    I have a oral sex on 21/11/2017
    Make a test on 26/12/2017
    Tested hiv ab/ag cmia non relative.
    Vdrl non relative.
    Hbsag also non relative.
    Anything im need to test again coz my side looking red. Sorry for broken english

    • I would consider you HIV test conclusive. Syphilis and hepatitis B will only be conclusive after 2-3 months, although risk of hepatitis B from oral sex is essentially zero.

      • Tq. Dr. Jonathan.
        So any recommended Syphilis test for me to do again?
        21/11/2017 – 12/1/2018 50days
        Got any blood test can consider syphilis?

  14. Hi Infectious Diseases Doctor,

    If a person in late Tertiary Stage without treatment, does Treponemal Tests(EIA,TPPA,FTA-ABS) still show positive result enough for a person to get treatment?