The different generations of ELISA

When HIV was identified as the virus that causes AIDS way back in 1986, there was a flurry of research looking into ways of identifying individuals who have been infected with the HIV virus. At the time, there was already a technique developed to detect various antibodies in the blood. This technique was called ELISA which stood for Enzyme Linked Immuno Sorbent Assay. This technique is based on the lock and key theory of antibodies. Basically, antibodies and antigens work like locks and keys. One key for one lock. One antibody fits one...

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HIV RNA / DNA PCR Test – Nucleic Acid Amplification Testing (NAAT)

HIV RNA / DNA PCR Test a.k.a Nucleic Acid Amplification Testing (NAAT) The HIV RNA test has revolutionized HIV screening especially for people at high risk. The test directly measures the amount of HIV virus in a person’s blood. This same test is also used to monitor how a person with HIV is responding to treatment. When used this way, it is called a Viral Load test. The HIV RNA test is deemed to be 100% conclusive if done after 10 to 12 days from the date of exposure.                   There...

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