Dr. Tan and Partners is one of the few approved Anonymous HIV Testing Centres in Singapore. A detailed write up on our service is available here.

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HIV P24 Ag/Ab Test, Duo Test, Combo Test at our Clinic

Updated information on Rapid Anonymous HIV Combo test at our Clinics. 


Info leaflet for SD Bioline Combo Test

Info leaflet for SD Bioline Combo Test

I have been getting a lot of question with regards to the Duo test or Combo test as it is also known as.

Let’s first get some basics right:

Duo Test = Combo Test = P24 Ag/Ab test

All these terms refer to the same thing. Basically it is a test that simultaneously tests for the HIV P24 Antigen and the HIV antibody.

HIV Prevention within 72 hours of exposure (PEP) – Click Here

The HIV P24 antigen is a small piece of protein that is found on the capsule of the HIV virus. When a person is infected with HIV, these bits of protein can be found floating in the blood. The HIV P24 test is a test that detects these bits of protein. This test was first developed as a HIV screening test but rapidly ran out of favor due to the development of more advanced NAAT tests. (For more details on NAAT testing please read: http://www.drtanandpartners.com/nucleic-acid-amplification-testing-naat/)The window period for P24 testing is also very small. This test alone is only accurate for between 3 and 6 weeks post exposure. So it is a test with very limited applications unless combined with the HIV antibody test. The presence of P24 Antigens in the blood indicates a recent HIV infection.

Click here for details on getting the Combo test at our Clinics

We all already know that the HIV antibody test becomes quite reliable at 4 weeks and as good as conclusive at 6 weeks. Although most health authorities still recommend a 3 months window period. So putting these 2 tests together gives you a very powerful tool to diagnose HIV infection in as little as 3 weeks. Personally I prefer to conduct the Combo test at 4 weeks because I believe that is when the level of P24 antigen peaks in the blood giving us maximum accuracy.

In our clinic, we use the SD Bioline Rapid HIV Combo Test 2.0. It is a rapid test that takes only 20 minutes for us to get results. We are also approved to conduct this test as part of the Anonymous HIV testing Program.

The test is conducted very much like the HIV antibody test. All we need is blood from a finger prick. (For more details on Anonymous HIV Testing at our clinic please read: http://www.drtanandpartners.com/anonymous-hiv-testing-at-our-clinic/)

SD Bioline HIV Combo Test showing a positive result (2 red bars)

SD Bioline HIV Combo Test showing a positive result (2 red bars)










SD Bioline Combo HIV test showing a positive result. Note the test line is very faint. This could be due to a very recent HIV infection or the test could be false positive (1% chance).

SD Bioline Combo HIV test showing a positive result. Note the test line is very faint. This could be due to a very recent HIV infection or the test could be false positive (1% chance).










Multicentre clinical trials have shown 100% sensitivity and more than 99% specificity.

This kit has been registered with Singapore’s Health Science Authority (HSA).

So the Combo Test has many advantages including:

1. Earlier detection of HIV infection.

2. Rapid (20 minutes)

3. Anonymous

At our clinic the Combo Test costs $150.

Click on the picture for the latest information on the HIV testing Window Period

Click on the picture for the latest information on the HIV testing Window Period










Click here for HIV Testing FAQ

More on HIV symptoms.

If you have any questions, visit our free online forum on sexual health, HIV and STDs.

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Click here to see the full range of our HIV/STD Services



Need more advice?

Come down to Our Clinics for a discussion with Our Doctors, or call our clinics for more information:

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2.) Bencoolen Street (+65 6884 4119)

3.) Novena Medical Centre (+65 6397 2095)

4.) Scotts Medical Centre (+65 6694 2348)

5.) Somerset – Orchard Building (+65 6262 0762)

6.) Katong – East Coast Road (+65 6635 2551)

Where to find us – here

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Selected clinics are open on Saturday and Sunday.

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All Our Doctors are Fully Certified and Trained to conduct specialised HIV Testing.

For lady patients who prefer female doctors, we have professional certified female Doctors to attend to your medical needs.

If you have any questions, visit our free online forum on sexual health, HIV and STDs.

If not read more about HIV symptoms.

Or find out more about Anonymous HIV testing at our clinics.


Can’t wait for 3 months to find out? Find out more about Anonymous Rapid HIV Combo test at our clinics.

If you feel that you are at risk, please contact us to make an appointment for the HIV testing/screening.

Our Andrology and Infection Centre at Novena Medical Center is open.

Call us at 6397 2095 now to book your treatment.

Related Topics:

Anonymous HIV testing at our Clinics

HIV – Understanding the Window Period

Cost of HIV/STD tests at our Clinics

Condom Broke

Getting HIV from Oral Sex

More on HIV symptoms

How long can HIV live outside the body?


About Dr. Tan
Dr. Tan graduated from the National University of Singapore in 2001. His residency was in the two largest public hospitals in Singapore; Tan Tock Seng Hospital and Singapore General Hospital.

Find the profiles of our panel of doctors.



Consultation STD Consultation – $60.00

Anonymous HIV Rapid Test only $20.00
(not inclusive of test)

STD screening
Standard HIV, Syphilis, Hepatitis B/C, Herpes Simplex Virus Type I and II $ 200.00
Urine – Chlamydia and Gonorrhoea PCR, Monilia, Trichomonas, NSU, Mycoplasma, Ureaplasma, Urine Culture, Candida $ 280.00
Urethritis and Vaginitis
Vaginal Swab – Chlamydia and Gonorrhoea PCR, Monilia, Trichomonas PCR, Gardnerella PCR, Candida PCR, Group B StrepNSU, Mycoplasma, Ureaplasma, Direct Microscopy, Candida, Vaginal swab culture $ 345.00
Comprehensive (Male) Blood – Syphilis, Hepatitis B/C, Herpes Simplex Virus Type I and IIUrine – Chlamydia and Gonorrhoea PCR, Monilia, Trichomonas, NSU, Mycoplasma, Ureaplasma, Urine Culture, Candida $ 390.00
Comprehensive (Female) Blood – Syphilis, Hepatitis B/C, Herpes Simplex Virus Type I and IIVaginal Swab – Chlamydia and Gonorrhoea PCR, Monilia, Trichomonas PCR, Gardnerella PCR, Candida PCR, Group B StrepNSU, Mycoplasma, Ureaplasma, Direct Microscopy, Candida, Vaginal swab culture $ 525.00
Individual STD Tests
Determine Syphilis test Rapid Syphilis Test $ 50.00
Determine Hepatitis B Test Rapid Hepatitis B Test $ 50.00
Herpes Simplex Virus PCR Herpes Simplex Virus Polymerase Chain Reaction (PCR) Test $ 190.00
Chlamydia and Gonoccocal PCR Chlamydia and Gonorrhea
Herpes Simplex Virus Type I IgG Antibody Herpes Type 1 $ 40.00
Herpes Simplex Virus Type II IgG Antibody Herpes Type 2 $ 40.00
Non-Specific Urethritis (NSU) Culture Mycoplasma, Ureaplasma $ 118.00
HIV test
HIV RNA/DNA Polymerase Chain Reaction (PCR) Test $600
Add $100 for expedited results
Rapid Fingerprick Blood HIV-1/2 P24 Antigen/Antibody Combo (HIV Duo Test) (4th generation!) $ 150.00
Rapid Oral Saliva HIV-1/2 Antibody Test (Oraquick) $ 50.00
Rapid Fingerprick Blood HIV-1/2 Antibody Test (Determine) $ 50.00
PEP $2000 – $2200


  1. Hi Doctor, is a HIV AG/AB test NEGATIVE at 65 days conclusive?

    • Dr. Julian Ng


      Generally, 4th Generation HIV tests (Ag/AB) is conclusive after 28 days post-exposure.

  2. superkacak

    Hi doctor, i got test at dtap kuala lumpur last feb (almost 7month post exposure) using sdbioline 4th gen. It was negative. The test just in 5-10 minute. Is the test conclusive?.
    Befpre that, I been test negative many time before using rapid and lab test. Can i move on.

    • Dr. Julian Ng


      If you have been tested on multiple occasions with the 4th generation HIV tests and were all negative esp 7 months after exposure, it is conclusive. Move on.

  3. Dear Doctor

    I am really having a difficult time due to my anxious nature. I went to a dentist in Tokyo for root canal treatment. He gave an injection to numb the tooth and did some serious dental work. During second visit after 8 days, he did some filling but no injection. After two days i.e. 10 days from first visit (when he gave an injection), I got fever for one night, one sided sore throat and swollen lymph nodes. I went to dentist, he said it is not due to dental work, but may be due to some viral or bacterial infection. Gave some antibiotics, it cleared way in one day. After finishing antibiotic course for 3 days, sore throat came again but not that sever, I started antibiotic again, it went away.

    I was terrified due to symptoms as they came together and were suggestive of ARS. I took HIV Ag/Ab test on 18 days from dental work (10 days from onset of symptoms), it came back negative. It gave me relief for some time, i started worrying again.

    After 50 days from dental work, I took HIV 1 RNA (Taqman PCR method detection limit 20 copies/ml), HIV antigen/antibody, Hep B surface antigen, Hep C antibody, Syphillis : All Negative.

    DO i need some more testing like at 90 days. What else can i do to establish that I am 100 percent HIV negative?

    Thank you very much in advance.

    Jo Jo

  4. Lembu miskin

    Hello dr tan. I had a small risk from blood of hiv+ person. It got contact to my wound (4 days old without scabbig). Tested at 45 days with eclia antibody test and 84 days with rapid prick combo test. Both result were negative. But i a have a whitish/dry skin lesion on my penis head that sometimes hurt and also swolenn lymph node at my neck that is sometimes painful. Could it be hiv related, and is my test conclusive? Should i need another testing?

    • Julian Ng


      Generally, if you test with HIV rapid Combo test (4th Generation) after 28 days, it is conclusive. However if you are concern about the whitish/dry skin lesion on the penis and lymph node, please consult a doctor for further evaluation as it is difficult to determine what it could be without seeing you. If you like, you can visit us at: http://www.drtanandpartners.com/where-to-find-us/

      • Lembu miskin

        The duo test should be as close as 28 days post exposure or is 84 days is good already? Can antigen be detected at 84 days?

  5. Hello doctor, I took 3 hiv duo test, with 41,61,92 all negative, with 110 days i notice a swollen noodle on my neck right side, sould I be afraid with HIV?

  6. Hi Doctor,

    I am experiencing fever, sore throat and fatigue, which is some of the symptoms of ARS, 2 weeks after a high risk encounter.

    If I take a 4th gen test now, would the test be able to detect p24 antigen or antibodies?

    Or should I wait till 28 days before taking the test ?

    Please advise and I will plan my trip to your clinic accordingly.


    • If you are having ARS symptoms, then the test would be able to pick up p24 antigen and/or antibody already. So we can do a test now if you are keen. However, for conclusive results we would still advise 28 days.

  7. Doc, can i have an answer please?
    The 4th gen combo test 9s valid after 3 years- 10 years-20 years from exposure Or it has to be sometime close to the exposure?

    Regarding the 4th gen combo, the antigen will not drop to undetected levels until there is enough detected antibodies right? Incase of seroconversion delayed so antigen will be detected and there will be no gaps?

    Thanks alot

    • It is valid any time after 28 days. It does not have to be close to the exposure. Antigen will be detected if there is no antibody.

  8. Greeting doctors,

    Is there a 3rd generatiob combo(duo ag/ab) test? Or its only a 4th generation?

  9. Greetings dr. Tan
    Could you please answer my question? Is hiv combo p24 Ag/Ab CMIA ‘please notice cmia’ is conclusive and reliable after 8 months or 2 years or even 10 years? Or this kind of test will detect only new infection?

    I really hope to get an answer as i read few things ib ur forum confused me, is this test is conclusive and im free of hiv if i took it anytime after 3 months? 1 year l 3 years etc…

  10. hello, doctor
    i tested hiv antibody only tests by using ‘Alere’ 24 , 75 , 80 , and 94 days after exposure were non reactive.

    I tested hiv by using hiv ag/ab combo test @ 115 days after exposure was non reactive.
    So, do i need another test?
    Are they conclusive?
    Sorry for my poor english skill.

  11. WonderGuy

    Does Multivitamin(eurobio),proplis,olive oil and black seed oil affect HIV test?

  12. Hello dr, my exposure is protected vaginal sex and unprotected oral sex(recive & giving)

    SD Bioline Combo

    20 days after exposure = Negative.
    24 days after exposure = Negative.
    27 days after exposure = Negative.

    Should I re test at 6 week? or 8 week?

  13. Tom cruise

    I tested hiv combo test after 115 days post expore.The result is non reactive and 0.245 unit of COI.The earlier i tested antibody only tests @ 24 80 and 94 days were non reactive.Do i need another test?Are they conclusive?

  14. Hellos doctor
    tomorrow I’m planning to do hiv test and it been like 5 weeks since the last exposure and i don’t know what test shull i do the new alere combo test or elisa ag ab which one is more accurate I’m really confused.
    Thank you

  15. Doctor I had ….

  16. Dear Doctor

    Thank you very much for running this forum and greeting from Japan. My exposure happened three months back in India. I visited a massage parlor for naked body to body massage. I was not involved in anal, vaginal or oral sex. I did few activities mentioned below:
    1. I sucked the nipple of the lady briefly 2-3 times.
    2. I rubbed her vagina with my finger and after 2-3 minutes , i masturbated myself using the same hand.
    3. I cleaned my penis using a towel which may had some infected fluid from previous customer.

    As I was terrified, I did some testing:
    Alere HIV combo @16, 26 and 43 days, blood was taken from arm and result was given after 20 minutes: All negative

    At 57th day, Laboratory based antigen/antibody test (CLIA) blood was taken from arm and result was given next day: Negative

    At 69th day, Laboratory based antigen/antibody test in Govt testing centre in Tokyo, blood was taken from arm and result was given next week : Negative

    At 90th day, Laboratory based HIV 1, 2 with P24 antigen (CMIA) test, blood was taken from arm: Negative

    At 106 days, Laboratory based antigen/antibody test (CLEIA), blood was taken from arm and result was given after 2 days : Negative.

    At 17th week after exposure, i developed soar throat with redness at the roof of the mouth. As i already did lot of internet surfing, I am worried that I may be a case of delayed conversation.

    Do I need to continue testing upto six months or do i need some advanced testing like NAT testing to make sure that I am 100 percent HIV free. This episode has entirely ripped off my personal and professional life and I am approximately hitting the point where I can loose my job and wife both. I tried taking professional help, but its not helping much…

    Please advice, I am sorry if my post is too long and annoyed you.

    • Your tests are conclusive and excessive – no HIV. I would advise seeking help from a psychiatrist.

      • Thank you very much doctor for your time in going through my query and giving response. I am a trying to get psychological help but language is a barrier here. Based on my risk and testing done afterwards, Can I be 100 percent (not 99 or 97 percent) sure that I am HIV negative? I am requesting you and I will be highly thankful if you can access my risk, whether it was a high risk or low risk?

        Thank you once again for doing good service.

  17. Hello doctor
    I had unprotected sex and I did the combo test in your clinic in malaysia the sd bioline ag/ab test. I did it on day 14,23 and 38 and they are negative shull I repeat again after 3 months or can I forget about it

  18. Christine

    I had the Alere Combo testing at 14 days, 29 days and 32 days with negative results.

    Can I trust these tests and feel confident I am negative even with symptoms?

  19. I recently had protected sex with an unknown women. The sorting part is I out on the condom after I had fingered her, with my hand that is full of timber virginal fluid.
    Also after sex I remove my condom and than I touch my penis.
    I had tested on day 14 with ALERE combo AG/AB test and it is negative. What does the result means? When is the earliest I could do a conclusive test? I am very worried as I for a pass few days I am having sweaty chest in the middle of the night.

  20. AnxiousMan

    Hi Doctor,
    I had protected sex with a masseuse on 8th Nov.
    On 21th Nov, day14 I day a HIV AG/AB ALERE rapid test and result negative. How accurate is the test?
    What is the chance it will turn positive on day 28?

  21. hi doc I from malaysia

    I doing sex (first time) after 15day 26/9/17 i test klinik kesihatan rapid test 3generations negatif,after 53day sex 1/11/17 I test private klinik ag/ab negatif,conclusive or not???my next appointment with klinik kesihatan 26/12 next test…possibility my next test to positive high or low???I relly stress now…everyday I chek my body…sorry my English bad..please answer my question doc

  22. Hi,
    Looking for some advice. I had unprotected insertive vaginal sex with a sex worker in the UK. I’m a circumcised male and there was no visible blood or trauma.
    I spoke to her 4 weeks post exposure and asked her regarding her hiv status. She said she is tested monthly, her last test being 2 weeks ago (so 2 weeks post exposure) and that she was negative. Said she had certificate and documents from the hospital. Obviously I’ve not seen this and can’t say what she said is 100% the truth.
    I’ve had the following tests:
    14 days post exposure – Alere HIV Combo (antibody/antigen test) (20 min result) – Negative
    21 days post exposure – Alere HIV Combo (anti/antigen) (20 min result) – negative
    28 days post exposure – Alere HIV Combo (anti/antigen) (20 min result) – negative
    35 days post exposure – Alere HIV Combo (anti/antigen) (20 min result) – Negative
    38 days post exposure – HIV DUO Lab test (antibody/antigen) (next day result) – negative
    42 days post exposure – Alere HIV Combo (anti/antigen) (20 min result) – negative

    The doctor said to me that since I’ve not had any obvious HIV symptoms and that all the tests that I have had are negative I can forget about hiv.

    Just wondering if that’s correct advice or am I right to still be anxious?

    Do I need to retest again at 8 or 12 weeks?


  23. alton cc

    Dear doctor
    I say not good english
    Today 50 days. I mean 7 week 1 days.I will re checked my hiv 1/2 ag and ab.. result 0.05.. when 35 days my ag and ab 0.07 after 15 days it reduse 0.02 i mean 0.05. Pls doctor say i recheck how days .

    I use condom sex with prostitute. But i touched prostitute clit 5 sec my left hand if i touched my panis left hand risk hiv effect? After sex when i open condom if some vaginal fluid touch my left hand and i wash water left hand my panis effect hiv?..after sex some precome on my panis head prostitute finger wipe this . This way any risk prostitute finger transmitted hiv… doctor i am not sleep no working panic for hiv. Pls give me a next suggestion. How days i recheck my hiv 1&2 ag and ab. Or no need test Pls answer me
    Thanks doctor

  24. Hello doctor,

    May I ask you a question.
    25.days, 42.days, 51.days fourth generation hiv duo ultra tests were negative.
    Can I trust that tests or need another duo at90 days.
    Many thanks .

    • Dr Elaine Loh

      Based on the description, I do not think you have HIV. If you are concerned, please get tested again 3 months from exposure.

  25. Hi dr tan

    I am janus from netherlands pls if you can help me out.90 days ago i had sex with a women hiv+ penetration was with a condom half on my penis i was drunk but i also fingered here and there was a lot of fluids on my hand all soaked after that i masturbated my self with the same hand stupid me. This encouter is for me a high risk but pls if you can help me out i have some question

    First lab test 12 days negative
    5 weeks 3 days lab test negative
    6.5 weeks alere rapid test negative
    9 weeks rapid test alere combo negative
    11 weeks lab test negative

    And i am still doubting a lot of anxiety and still all sympoms of hiv infection

    Can i say i was verry lucky or should i make another test at 90 days wich is tomorow

  26. Hi doctor,
    25 days, 42 days and 51.days vidas hiv duo ultra 4th generation tests was negative. Is it conclusive or I need another tests.?
    Many thanks.

  27. hi sir

    after protected anal and unprotected oral on a girl
    i made a test HIV ag/ab 1+2 and the result came 0.58
    i did the test before and the result was 0.12,0.14,0.10

    the result has been done after 29 days but my question why is that the number getting higher ?

    thank you sir 🙂

    • There are other similar non-HIV antibodies that can be detected by the test, and a margin of error allowed for detection. As long as it does not cross the threshold for “reactive” you are fine and do not have to worry. If you have further concerns, please speak to the healthcare providers that administered your testing.

      • than you sir

        1- you mean by threshold is >=1 ??
        2- should i consider myself conservative negative ?

        thank you Dr.

  28. Hello. Ive received answers from alot of blogs and paid medical forums saying that i have no risk. Its regarding hiv transmission on my shin (im afraid that vaginal fluid may have entered the skin) eventhough the skin upon inspection is intact. im worried about microwounds.

    So when can i test? And how much will the test cost?

  29. Hi doc

    Can we take a hiv duo test on 28th day or it should be after 28 days.

    I received a handjob from a massage therapist which was on August 27, 2017

    Test is for my peace of mind

  30. Dear Dr…Is 4th gen duo test conclusive at 28 days?….Some specialists saying it’s conclusive at 6 weeks…Some saying it’s at 3 months….It’s confusing….Please tell me why different opinions…..Which one we should follow.

    • Our opinion is that it is conclusive at 28 days. At the end of the day we are also just another expert, so the best place to go is your own medical doctor.

  31. Sorry to add on, but I had a ache and sore feeling on my gut are and the area between my butt and the testicles on the 1st week. The std clinic doctor told me to find another doctor for that. Could it be due to me trying to poop very hard for a few times while I was overseas? I visited the doctor he gave me antibiotics and it was gone after a few days. But at the 6th week it come back again. Only for a day though

  32. Had protected oral sex with sex worker(mouth to penis). Went to get tested for comprehensive urine check after 1 week,results were negative. Then had a Duo combo rapid test at 29 days after exposure. Result tested negative but there were some blood stains on it,but don’t think I saw the lines that state I am HIV positive,the nurse there told me that I tested negative. Do you reckon I need another test? Or am I cleared? Thanks

  33. I go for HIV duo test after 27 days..it’s come negative..my results is

  34. Hi Dr, i tested negative at 29 days post exposure with 4th generation ag/ab hiv test. My question is how you are so sure that it is conclusive after 28 days? because UK guidelines and CDC guidelines all stated that the result at 4 weeks only 95% accurate and we need to retest again at 6 weeks and 3 months. They said some people need more time to develop antigen n antibody

  35. My duo test on 24 days, 30days. 38ays conclusive for hiv. And other std check at six week conclusive at 40days conclusive?

    • The HIV tests are conclusive. The other STD check will depend on what was checked. But I would say the majority of STDs can be picked up by 40 days post-exposure.

  36. Hi Dr, i have done 4th generation ag/ab combo test with gribbles company in malaysia at 21 days(3 weeks) post exposure and it turn out negative. Can i rely on the result and expect it wouldn’t change in further test?

  37. Hi dr Tan, Went to your anonymous clinic last week and did the alere hiv combo test. Did the test at day 34 after exposure. Would like you to confirm that the negative test is a confirmation that I have no hiv. Thank you.

  38. Hi DR.. I had sex with a sex worker 2 weeks before.. I did CMIA test on 15th day and the result is non-reactive. My question is how many percentage is the result is accurate? Thank you

    • If your test was 4th generation and included antigen and antibody, it is a very good indication, but still not 100% conclusive. Please repeat a 4th gen test at 28 days or later.

  39. dr, just follow up, i just done a combo test after my exposed 21days and currently i have hives on my skin (around 1 weeks). the 21days combo test is negative and can i say that the rash that i hving is not related to ars?

  40. Hi dr, 2 weeks ago i was exposed to csw without any sex and oral. I was hj myself with condom. Currently i was hving a skin rash something like hives and will go for cpmbo test at your place.
    1. Hives is the ars?
    2. I was hj myself and the girl may touch my body is tht risk?
    3. Combo test at 21days is good indicate for my case as without any penetration?
    4. The ski rash like bump, become red then will slowly disappear and itch at night.is this the skin rash ars? It look like a large bump.

    • From the exposure you described, the risk of HIV transmission is zero. Hives can be caused by many things. If you are still concerned, please come down to our Robertson Walk clinic for a consult and evaluation of your rashes.

      • hi dr thks for reply,
        but hives can be the ars? and 21 days from exposed can i get a combo test to move on?

  41. Hi Dr.
    I had unprotected sex with my new girl friend on Apr 12 this year. She’s VDRL and Syphilis test March 7 was non reactive. I don’t know she’s current Hiv status but according to the employment last year June was non reactive. Any way I take Hiv p24 antigen test at 28days now waiting for the result. This antigen test result is whether conclusive or not. And then I already have plan to come your clinic for HIV and STD test screening. Please help me reply thanks.

    • The combo antigen+antibody test would be conclusive at 28 days. But you are welcome to come by our clinic for further consult and testing.


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